Extension of Genetic Marker List Using Unnatural Amino Acid System: An Efficient Genomic Modification Strategy in Escherichia coli.
Genetic manipulations together with chromosome engineering are important methods used to restructure cell metabolism. Lambda/Red (Ī»/Red)-mediated recombination is probably the most generally utilized strategy for chromosomal modulation inĀ Escherichia coli. However, the effectivity of this technique is considerably hampered by the laborious elimination of the selectable markers. To overcome the issue, the mixing helper plasmid was … Read more