Cusabio Arachis hypogaea Recombinant

Product specifications

  • Size: 10ug or 50ug
  • Format: Lyophilized powder
  • Formulation: lyophilized from a 0.45 µm filtered solution in 20 mM NaH2PO4 (pH 7.4), 0.5 M NaCl, 800 mM imidazole.
  • Purification: His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
  • Concentration: Determined by Bio-Rad Protein Assay Dye Reagent Concentrate.
  • Purity: >85%
  • Reconstitution: Briefly invert the vial and bring the contents to the bottom before opening. Reconstitution at 0.5~1.0mg/mL with sterile deionized water is recommended.
  • Purity determined by: SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
  • Recommended applications: Protein-protein interaction, Post-translational modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

Abstract

Peanuts are a multipurpose oilseed legume, offering benefits in many ways. Apart from the beneficial effects of the peanut plant on soil quality, peanut seeds are nutritious and important from a medical and economic point of view. In this review, information is provided on the origin of the peanut and its domestication. Peanuts are rich in bioactive components, including phenols, flavonoids, polyphenols, and resveratrol. In addition, the participation of peanuts in biological nitrogen fixation is very significant.

Recent reports on Arachis hypogaea Recombinant responses and N2 fixation capacity in response to abiotic stresses, including drought, salinity, heat stress, and iron deficiency in calcareous soils, have been incorporated. As a biotechnological note, recent advances in the development of transgenic peanut plants are also highlighted. In this context, the regulation of transcriptional factors and gene transfer for the development of stress-tolerant peanut genotypes are of paramount importance. Above all, this review signifies the importance of peanut cultivation and human consumption in the face of global climate change to maintain food security.

Fountain

  • Genetic symbols: Ara h 7
  • Species: Arachis hypogaea
  • Access number: Q9SQH1
  • Expressed Region: Thr22-Leu160
  • Protein name and synonyms: Ara h 7, Ara h 7 allergen

Preparation

  • Expression System: E.Coli
  • Conjugation/Tag: N-terminal histidine tag
  • Molecular weight (kDa): 18

Stability and Storage

  • Shipping: Ice packs
  • Storage/Stability: Upon arrival, lyophilized protein can be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store desiccated below -20°C in a manual defrost the freezer. After reconstitution, the protein can be stored for 2 weeks under sterile conditions at -20°C. For long-term storage, it is recommended to make suitable aliquots and store them at -80°C. Avoid repeated cycles of freezing and thawing.